Great Circle Navigation with Vectorial Methods

The present paper is concerned with the solution of a series of practical problems relevant to great circle navigation, including the determination of the true course at any point on the great circle route and the determination of the lateral deviation from a desired great circle route. Intersection between two great circles or between a great circle and a parallel is also analyzed. These problems are approached by means of vector analysis, which yields solutions in a very compact form that can be computed numerically in a very straightforward manner. This approach is thus particularly appealing for performing computer-aided great circle navigation

Repetitive low-frequency stimulation reduces epileptiform synchronization in limbic neuronal networks.

Deep-brain electrical or transcranial magnetic stimulation may represent a therapeutic tool for controlling seizures in patients presenting with epileptic disorders resistant to antiepileptic drugs. In keeping with this clinical evidence, we have reported that repetitive electrical stimuli delivered at approximately 1 Hz in mouse hippocampus-entorhinal cortex (EC) slices depress the EC ability to generate ictal activity induced by the application of 4-aminopyridine (4AP) or Mg2+-free medium (Barbarosie, M., Avoli, M., 1997. CA3-driven hippocampal–entorhinal loop controls rather than sustains in vitro limbic seizures. J. Neurosci. 17, 9308–9314.). Here, we confirmed a similar control mechanism in rat brain slices analyzed with field potential recordings during 4AP (50 μM) treatment. In addition, we used intrinsic optical signal (IOS) recordings to quantify the intensity and spatial characteristics of this inhibitory influence. IOSs reflect the changes in light transmittance throughout the entire extent of the slice, and are thus reliable markers of limbic network epileptiform synchronization. First, we found that in the presence of 4AP, the IOS increases, induced by a train of electrical stimuli (10 Hz for 1 s) or by recurrent, single-shock stimulation delivered at 0.05 Hz in the deep EC layers, are reduced in intensity and area size by low-frequency (1 Hz), repetitive stimulation of the subiculum; these effects were observed in all limbic areas contained in the slice. Second, by testing the effects induced by repetitive subicular stimulation at 0.2–10 Hz, we identified maximal efficacy when repetitive stimuli are delivered at 1 Hz. Finally, we discovered that similar, but slightly less pronounced, inhibitory effects occur when repetitive stimuli at 1 Hz are delivered in the EC, suggesting that the reduction of IOSs seen during repetitive stimulation is pathway dependent as well as activity dependent. Thus, the activation of limbic networks at low frequency reduces the intensity and spatial extent of the IOS changes that accompany ictal synchronization in an in vitro slice preparation. This conclusion supports the view that repetitive stimulation may represent a potential therapeutic tool for controlling seizures in patients with pharmacoresistant epileptic disorders

Cholesterol depletion inhibits synaptic transmission and synaptic plasticity in rat hippocampus.

Several neurodegenerative disorders are associated with impaired cholesterol homeostasis in the nervous system where cholesterol is known to play a role in modulating synaptic activity and stabilizing membrane microdomains. In the present report, we investigated the effects of methyl-beta-cyclodextrin-induced cholesterol depletion on synaptic transmission and on the expression of 1) paired-pulse facilitation (PPF); 2) paired-pulse inhibition (PPI) and 3) long-term potentiation (LTP) in the CA1 hippocampal region. Results demonstrated that cyclodextrin strongly reduced synaptic transmission and blocked the expression of LTP, but did not affect PPF and PPI. The role of glutamatergic and GABAergic receptors in these cholesterol depletion-mediated effects was evaluated pharmacologically. Data indicate that, in cholesterol depleted neurons, modulation of synaptic transmission and synaptic plasticity phenomena are sustained by AMPA-, kainate-and NMDA-receptors but not by GABA-receptors. The involvement of AMPA-and kainate-receptors was confirmed by fluorimetric analysis of intracellular calcium concentrations in hippocampal cell cultures. These data suggest that modulation of receptor activity by manipulation of membrane lipids is a possible therapeutic strategy in neurodegenerative disease

Impaired Activation of CA3 Pyramidal Neurons in the Epileptic Hippocampus.

We employed in vitro and ex vivo imaging tools to characterize the function of limbic neuron networks in pilocarpine-treated and age-matched, nonepileptic control (NEC) rats. Pilocarpine-treated animals represent an established model of mesial temporal lobe epilepsy. Intrinsic optical signal (IOS) analysis of hippocampal-entorhinal cortex (EC) slices obtained from epileptic rats 3 wk after pilocarpine-induced status epilepticus (SE) revealed hyperexcitability in many limbic areas, but not in CA3 and medial EC layer III. By visualizing immunopositivity for FosB/DeltaFosB-related proteins which accumulate in the nuclei of neurons activated by seizures we found that: (1) 24 h after SE, FosB/DeltaFosB immunoreactivity was absent in medial EC layer III, but abundant in dentate gyrus, hippocampus proper (including CA3) and subiculum; (2) FosB/DeltaFosB levels progressively diminished 3 and 7 d after SE, whereas remaining elevated (p < 0.01) in subiculum; (3) FosB/DeltaFosB levels sharply increased 2 wk after SE (and remained elevated up to 3 wk) in dentate gyrus and in most of the other areas but not in CA3. A conspicuous neuronal damage was noticed in medial EC layer III, whereas hippocampus was more preserved. IOS analysis of the stimulus-induced responses in slices 3 wk after SE demonstrated that IOSs in CA3 were lower (p < 0.05) than in NEC slices following dentate gyrus stimulation, but not when stimuli were delivered in CA3. These findings indicate that CA3 networks are hypoactive in comparison with other epileptic limbic areas. We propose that this feature may affect the ability of hippocampal outputs to control epileptiform synchronization in EC

Size and albedo distributions of asteroids in cometary orbits using WISE data

Context. Determining whether asteroids in cometary orbits (ACOs) are dormant or extinct comets is relevant for understanding the end-states of comets and the sizes of the comet population. Aims. We intend to study the value distributions of effective diameter (D), beaming parameter (η), and visible geometric albedo (pV) of ACO populations, which can be derived from NASA's Wide-field Infrared Explorer (WISE) observations, and we aim to compare these with the same, independently determined properties of the comets. Methods. The near-Earth asteroid thermal model (NEATM) is used with WISE data and the absolute magnitude (H) of the ACOs to compute the D, pV and η. Results. We obtained D and pV for 49 ACOs in Jupiter family cometary orbits (JF-ACOs) and 16 ACOs in Halley-type cometary orbits (Damocloids). We also obtained the infrared beaming parameter η for 45 of them. All but three JF-ACOs (95% of the sample) present a low albedo compatible with a cometary origin. The pV and η distributions of both ACO populations are very similar. For the entire sample of ACOs, the mean geometric albedo is p V = 0.05±0.02, (p V = 0.05±0.01 and p V = 0.05±0.02 for JF-ACOs and for Damocloids, respectively) compatible with a narrow albedo distribution similar to that of the Jupiter family comets (JFCs), with a p V ∼ 0.04. The mean beaming parameter is η = 1.0±0.2. We find no correlations between D, pV, or η. We also compare the cumulative size distribution (CSD) of ACOs, Centaurs, and JFCs. Although the Centaur sample contains larger objects, the linear parts in their log-log plot of the CSDs presents a similar cumulative exponent (β = 1.85 ± 0.30 and 1.76 ± 0.35, respectively). The CSD for Damocloids presents a much shallower exponent β = 0.89 ± 0.17. Conclusions. The pV- and η-value distributions of ACOs are very similar to those of JF comet (JFCs) nuclei. The ACOs in Tancredi's list are the best possible candidates to be dormant/inactive comets. The CSD for JF-ACOs is shallower and shifted towards larger diameters with respect to the CSD of active JFCs, which suggests that the mantling process has a size dependency whereby large comets tend to reach an inactive stage faster than small comets. Finally, the population of JF-ACOs is comparable in number with the population of JFCs, although there are more tens-km JF-ACOs than JFCs

Reduced GABA(B) receptor subunit expression and paired-pulse depression in a genetic model of absence seizures

Neocortical networks play a major role in the genesis of generalized spike-and-wave (SW) discharges associated with absence seizures in humans and in animal models, including genetically predisposed WAG/Rij rats. Here, we tested the hypothesis that alterations in GABAB receptors contribute to neocortical hyperexcitability in these animals. By using Real-Time PCR we found that mRNA levels for most GABAB(1) subunits are diminished in epileptic WAG/Rij neocortex as compared with age-matched non-epileptic controls (NEC), whereas GABAB(2) mRNA is unchanged. Next, we investigated the cellular distribution of GABAB(1) and GABAB(2) subunits by confocal microscopy and discovered that GABAB(1) subunits fail to localize in the distal dendrites of WAG/Rij neocortical pyramidal cells. Intracellular recordings from neocortical cells in an in vitro slice preparation demonstrated reduced paired-pulse depression of pharmacologically isolated excitatory and inhibitory responses in epileptic WAG/Rij rats as compared with NECs; moreover, paired-pulse depression in NEC slices was diminished by a GABAB receptor antagonist to a greater extent than in WAG/Rij rats further suggesting GABAB receptor dysfunction. In conclusion, our data identify changes in GABAB receptor subunit expression and distribution along with decreased paired-pulse depression in epileptic WAG/Rij rat neocortex. We propose that these alterations may contribute to neocorticalhyperexcitability and thus to SW generation in absence epilepsy

Events with Isolated Charged Leptons and Large Missing Transverse Momentum at HERA

Striking events with isolated charged leptons, large missing transverse momentum and large transverse momentum of the hadronic final state were observed at the electron proton collider HERA in a data sample corresponding to a luminosity of about 130 pb-1. The H1 collaboration observed 11 events with isolated electrons or muons and with transverse momentum above 25 GeV. Only 3.4+-0.6 events were expected from Standard Model (SM) processes. Six of these events have a transverse momentum of greater than 40 GeV, while 1.3+-0.3 events were expected. The ZEUS collaboration observed good agreement with the SM. However, ZEUS found two events with a similar event topology, but tau leptons instead of electrons or muons in the final state. Only 0.2+-0.05 events were expected from SM processes. For various hypotheses the compatibility of the experimental results was investigated with respect to the SM and with respect to possible explanations beyond the SM. Prospects for the high-luminosity HERA-II data taking period are given